Ivermectin Pharmacology
Clinical Particulars
Ivermectin is an avermectin structurally related to milbemycins such as Moxidectin. Avermectins are naturally occurring compounds generated as fermentation products by Streptomyces avermitilis, a soil actinomycete.
Pharmacokinetics
Mechanism of Action
Invertebrate Nerve Transmission Disruption: Ivermectin targets g-aminobutyric acid (GABA) receptors and ivermectin-sensitive glutamate-gated chloride channel receptors, only found in invertebrates.
Chloride Influx: Avermectins interfere with parasite nerve transmission by indirectly opening chloride channels in the post-synaptic membrane of invertebrate nerve and muscle cells. This causes an influx of chloride ions into the cells, leading to hyperpolarisation and subsequent paralysis of invertebrate neuromuscular systems.
Glutamate Enhancement: Pharmacological action is mostly through enhancing the effects of glutamate at the glutamate-gated chloride channels specific to protostome invertebrates. Parasite specificity occurs in most veterinary species, as typical mammalian patients do not possess protostome-specific glutamate-gated chloride channels (Wolstenholme, 2012).
Summary of Applications
Endectocide: Active against many immature and mature nematodes and arthropods. Often employed as a general, non-specific antiparasitic therapy. Mixed target species activity. Used extensively. Some species and individuals demonstrate extreme sensitivity/toxicity issues.
Arthropod Activity: Active against a wide range of immature and mature arthropods, causing flaccid paralysis of parasites.
Nematode Activity: Avermectins are effective against a wide range of nematode species and developmental stages,.
Cestode Activity: Ivermectin is ineffective against trematodes and cestodes as these species have no macrocyclic lactone binding sites.
Pharmacodynamics
Metabolism
Hepatic: Metabolized in the liver via oxidative pathways (Martin and others 2020).
Elimination
Faecal: Primarily excreted in the faeces. Less than 5% of the drug (as parent compound or metabolite) is excreted in the urine. Exctreted ivermectin and its metabolites remain toxic to dung-feeding insects but not birds, plants, and earthworms (Lumaret and others 2012; Martin and others 2020).
Precautions
Adverse Effects
General Adverse Effects | Neurotoxicity is possible in any patient: Signs of neurotoxicity include but are not limited to ataxia, mydriasis, blindness, tremors, vomiting, seizures, and cardiovascular and respiratory depression. Chronic and subchronic signs of neurotoxicity are also reported in some canine patients after prolonged dosing (Bissonnette et al., 2009).
Ivermectin | Species-Specific Adverse Effects
Dogs | Neurotoxicity: Collapse coma and death are possible. Signs of toxicity in dogs include GCS> 10, vomiting, hypersalivation, dilated pupils, ataxia and apparent blindness (An absence of both menace responses and pupillary light reflexes). Animals may present panting and depression, hypersalivation, hyperaesthesia, tremors and twitching. Normocardia and normothermia are possible, but some animals may be pyrexic or hypothermic and very depressed, and comatose animals may show abnormal heart rate and rhythm (Bissonnette et al., 2009; Bates et al., 2013; Becker and Young, 2017).
Rodents
Mice and Rats | Neurotoxicity: Ivermectin may cause neurologic toxicity at therapeutic doses (i.e. less than 0.5 mg/kg).
Reptiles
Chelonians: Flaccid paralysis and death have been reported in many chelonian species, including red-footed tortoises (Geochelone carbonaria), leopard tortoises (Geochelone pardalis) (Teare and Bush 1983)
Skinks, Indigo Snakes, and crocodilians: Species that have suffered profound fatal neurotoxicity (Petritz & Chen 2018).
Avians
Orange-cheeked Waxbill Finches | Budgerigars: Sensitive Species have suffered profound fatal neurotoxity (Petritz & Chen 2018).
Aquatic Animals
Fish and other Aquatic Animals: Ivermectin is highly toxic to aquatic animals, so it should never be released into aquatic environments. However, it binds heavily to soil and rarely drains from land into water courses.
Contraindications
General Contraindications
Hypersensitivity: Do not administer to animals with known hypersensitivity to the drug
Species-Specific Contraindications
Dogs | ABCB1 (MDR-1) mutation: Do not administer to dogs with a strong chance of MDR1 gene mutation (e.g., All types of collies, Australian Shepherd Dogs, Shelties, Long-haired whippets, breeds with “white feet”). These dogs are likely to suffer significant adverse effects. Neurotoxicity is possible. ABCB1 (MDR-1) mutation is a relative contraindication (higher dosages should be avoided and an alternative drug used)
Dogs | Neonates: Ivermectin is not recommended for use in puppies < 6 weeks of age
Dogs | Active Heartworm Infestation: Ivermectin is not recommended for use in dogs without a current negative heartworm test.
Reptiles
Chelonians | Indigo Snakes | Crocodilians | Skinks: Because of serious adverse effects, including neurotoxicity and death, the use in these species is not recommended.
Avians
Orange-cheeked Waxbill Finches | Budgerigars: These species are not recommended because of serious adverse effects, including neurotoxicity and death.
Aquatic Animals
Fish and other Aquatic Animals: Ivermectin is highly toxic to aquatic animals, so It should never be released into aquatic environments. However, it binds heavily to soil and rarely drains from land into water courses.
Potentially Significant Interactions
General interactions
Benzodiazepines: Effects may be potentiated by Ivermectin; use together is not advised in humans.
Drug Specific Interactions
Amiodarone: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Carvedilol: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Clarithromycin: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Ciclosporin: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Diltiazem: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Erythromycin: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Itraconazole: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Ketoconazole: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Loperamide: Caution is advised if other drugs can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation. This allows Loperamide (a P-glycoprotein substrate) to penetrate the CNS and cause profound sedation.
Phenobarbital: Do not use in seizures of toxic causes where the clinical signs are mediated through the GABA channels (Ivermectin and Moxidectin toxicity) as this may exacerbate the seizures
Quinidine: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Spironolactone: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Tamoxifen: Caution is advised if using other drugs that can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation
Verapamil: Caution is advised if other drugs can inhibit P-glycoprotein, especially in dogs at risk for MDR1-allele mutation.
Reproduction
Pregnancy | Relative Safety: Manufacturers' data indicates no significant adverse effects of Ivermectin on pregnancy in dogs, horses, cattle, or swine. However, high doses appeared to have a teratogenic effect in rats and rabbits.
Lactation | Safe: Manufacturers' data indicates that Ivermectin is excreted into breast milk but is unlikely to influence the nursing offspring when used in therapeutic doses.
Last Update | 220720
5 | Monitoring
Adverse Effects
Suspected or Proven
Vital Signs
HR, RR, T etc
Perfusion parameters
Body Condition Score
Physiological Changes
Cardiac Function
Digestive Function
Hepatic Function
Renal Function
Respiratory Function
Neurologal Function
Disease Stage
Continue to stage the primary morbidity.
Clinical Efficacy
Monitor the patient concerning treatment goals.
Set a plan to determine the end of a need for treatment.
Availability
A significant number of products are listed at the VMD PID | https://www.vmd.defra.gov.uk/productinformationdatabase/search
A significant number of products are listed at the FDA PID | https://animaldrugsatfda.fda.gov/adafda/views/#/search
A significant number of products are listed at the EUD Medicines PID | https://www.ema.europa.eu/en/medicines
Identifiers
Pharmacotherapeutic Group(s): Avermectines
ATC Code(s): P02CF01
ATC Vet Code(s): QP02CF01
The WHO ATC/DDD Index 2022 | https://www.whocc.no/atc_ddd_index
The WHO ATCvet Index 2021 | https://www.whocc.no/atcvet/atcvet_index/occ.no/atcvet/atcvet_index/
Evidence Base
Al-Azzam, S.I., Fleckenstein, L., Cheng, K.-J., Dzimianski, M.T., McCall, J.W., 2007. Comparison of the pharmacokinetics of moxidectin and ivermectin after oral administration to beagle dogs. Biopharmaceutics & Drug Disposition 28, 431–438. https://doi.org/10.1002/bdd.572
Ballent, M., Lifschitz, A., Virkel, G., Mate, L., Lanusse, C., 2010. Pretreatment with the inducers rifampicin and phenobarbital alters ivermectin gastrointestinal disposition. Journal of Veterinary Pharmacology and Therapeutics 33, 252–259. https://doi.org/10.1111/j.1365-2885.2009.01129.x
Bates, N., Chatterton, J., Robbins, C., Wells, K., Hughes, J., Stone, M., Campbell, A., 2013. Lipid infusion in the management of poisoning: a report of 6 canine cases. Veterinary Record 172, 339–339. https://doi.org/10.1136/vr.101036
Becker, M.D., Young, B.C., 2017. Treatment of severe lipophilic intoxications with intravenous lipid emulsion: a case series (2011–2014). VMRR 8, 77–85. https://doi.org/10.2147/VMRR.S129576
Bissonnette, S., Paradis, M., Daneau, I., Silversides, D.W., 2009. The ABCB1-1Delta mutation is not responsible for subchronic neurotoxicity seen in dogs of non-collie breeds following macrocyclic lactone treatment for generalized demodicosis. Vet Dermatol 20, 60–66. https://doi.org/10.1111/j.1365-3164.2008.00731.x
Bloomquist, J.R., 2003. Chloride channels as tools for developing selective insecticides. Arch Insect Biochem Physiol 54, 145–156. https://doi.org/10.1002/arch.10112
Bloomquist, J.R., 1993. Toxicology, mode of action and target site-mediated resistance to insecticides acting on chloride channels. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology 106, 301–314. https://doi.org/10.1016/0742-8413(93)90138-B
Bundschuh, M., Hahn, T., Ehrlich, B., Höltge, S., Kreuzig, R., Schulz, R., 2016. Acute Toxicity and Environmental Risks of Five Veterinary Pharmaceuticals for Aquatic Macroinvertebrates. Bull Environ Contam Toxicol 96, 139–143. https://doi.org/10.1007/s00128-015-1656-8
Campbell, W.C., Benz, G.W., 1984. Ivermectin: a review of efficacy and safety. Journal of Veterinary Pharmacology and Therapeutics 7, 1–16. https://doi.org/10.1111/j.1365-2885.1984.tb00872.x
Chen, L.-J., Sun, B.-H., Qu, J.P., Xu, S., Li, S., 2013. Avermectin induced inflammation damage in king pigeon brain. Chemosphere 93, 2528–2534. https://doi.org/10.1016/j.chemosphere.2013.09.058
Chittrakarn, S., Janchawee, B., Ruangrut, P., Kansenalak, S., Chethanond, U., Kobasa, T., Thammapalo, S., 2009. Pharmacokinetics of ivermectin in cats receiving a single subcutaneous dose. Research in Veterinary Science 86, 503–507. https://doi.org/10.1016/j.rvsc.2008.08.005
Clarke, D.L., Lee, J.A., Murphy, L.A., Reineke, E.L., 2011. Use of intravenous lipid emulsion to treat ivermectin toxicosis in a Border Collie. Journal of the American Veterinary Medical Association 239, 1328–1333. https://doi.org/10.2460/javma.239.10.1328
Diao, H., Cheng, N., Zhao, Y., Xu, H., Dong, H., Thamm, D.H., Zhang, D., Lin, D., 2019. Ivermectin inhibits canine mammary tumor growth by regulating cell cycle progression and WNT signaling. BMC Vet Res 15, 276. https://doi.org/10.1186/s12917-019-2026-2
Edwards, G., 2003. Ivermectin: does P-glycoprotein play a role in neurotoxicity? Filaria J 2, S8. https://doi.org/10.1186/1475-2883-2-S1-S8
Epstein, S.E., Hollingsworth, S.R., 2013. Ivermectin-induced blindness treated with intravenous lipid therapy in a dog. Journal of Veterinary Emergency and Critical Care 23, 58–62. https://doi.org/10.1111/vec.12016
Fernandez, A.L., Lee, J.A., Rahilly, L., Hovda, L., Brutlag, A.G., Engebretsen, K., 2011. The use of intravenous lipid emulsion as an antidote in veterinary toxicology. J Vet Emerg Crit Care (San Antonio) 21, 309–320. https://doi.org/10.1111/j.1476-4431.2011.00657.x
Ferreira Bulhosa, L., Estrela-Lima, A., da Silva Solcà, M., Diniz Gonçalves, G.S., Larangeira, D.F., de Pinho, F.A., Barrouin-Melo, S.M., 2020. Vincristine and ivermectin combination chemotherapy in dogs with natural transmissible venereal tumor of different cyto-morphological patterns: A prospective outcome evaluation. Anim Reprod Sci 216, 106358. https://doi.org/10.1016/j.anireprosci.2020.106358
González Canga, A., Sahagún Prieto, A.M., José Diez Liébana, M., Martínez, N.F., Vega, M.S., Vieitez, J.J.G., 2009. The pharmacokinetics and metabolism of ivermectin in domestic animal species. The Veterinary Journal 179, 25–37. https://doi.org/10.1016/j.tvjl.2007.07.011
Gwaltney-Brant, S., Meadows, I., 2012. Use of Intravenous Lipid Emulsions for Treating Certain Poisoning Cases in Small Animals. Veterinary Clinics of North America: Small Animal Practice, Common Toxicologic Issues in Small Animals 42, 251–262. https://doi.org/10.1016/j.cvsm.2011.12.001
Held, S., Gramer, I., Hassdenteufel, E., Neiger, R., Geyer, J., 2012. Lipid-infusion therapy of avermectin-induced neurotoxicosis in two dogs with homozygous nt230(del4) MDR1 mutation. Kleintierpraxis 57, 313–319. https://doi.org/10.2377/0023-2076-57-313
Hugnet, C., Bentjen, S.A., Mealey, K.L., 2004. Frequency of the mutant MDR1 allele associated with multidrug sensitivity in a sample of collies from France. Journal of Veterinary Pharmacology and Therapeutics 27, 227–229. https://doi.org/10.1111/j.1365-2885.2004.00585.x
Jiang, L., Wang, P., Sun, Y.-J., Wu, Y.-J., 2019. Ivermectin reverses the drug resistance in cancer cells through EGFR/ERK/Akt/NF-κB pathway. J Exp Clin Cancer Res 38, 265. https://doi.org/10.1186/s13046-019-1251-7
Jourdan, G., Boyer, G., Raymond-Letron, I., Bouhsira, E., Bedel, B., Verwaerde, P., 2015. Intravenous lipid emulsion therapy in 20 cats accidentally overdosed with ivermectin. J Vet Emerg Crit Care (San Antonio) 25, 667–671. https://doi.org/10.1111/vec.12371
Juarez, M., Schcolnik-Cabrera, A., Dueñas-Gonzalez, A., 2018. The multitargeted drug ivermectin: from an antiparasitic agent to a repositioned cancer drug. Am J Cancer Res 8, 317–331.
Kidwell, J.H., Buckley, G.J., Allen, A.E., Bandt, C., 2014. Use of IV Lipid Emulsion for Treatment of Ivermectin Toxicosis in a Cat. Journal of the American Animal Hospital Association 50, 59–61. https://doi.org/10.5326/JAAHA-MS-5951
Lumaret, J.-P., Errouissi, F., Floate, K., Römbke, J., Wardhaugh, K., 2012. A review on the toxicity and non-target effects of macrocyclic lactones in terrestrial and aquatic environments. Curr Pharm Biotechnol 13, 1004–1060. https://doi.org/10.2174/138920112800399257
Markowska, A., Kaysiewicz, J., Markowska, J., Huczyński, A., 2019. Doxycycline, salinomycin, monensin and ivermectin repositioned as cancer drugs. Bioorganic & Medicinal Chemistry Letters 29, 1549–1554. https://doi.org/10.1016/j.bmcl.2019.04.045
Martin, R.J., Robertson, A.P., Choudhary, S., 2021. Ivermectin: An Anthelmintic, an Insecticide, and Much More. Trends Parasitol 37, 48–64. https://doi.org/10.1016/j.pt.2020.10.005
Martinez, M., Modric, S., Sharkey, M., Troutman, L., Walker, L., Mealey, K., 2008. The pharmacogenomics of P-glycoprotein and its role in veterinary medicine. Journal of Veterinary Pharmacology and Therapeutics 31, 285–300. https://doi.org/10.1111/j.1365-2885.2008.00964.x
Mealey, K.L., 2013. Adverse Drug Reactions in Veterinary Patients Associated with Drug Transporters. Veterinary Clinics of North America: Small Animal Practice, Clinical Pharmacology and Therapeutics 43, 1067–1078. https://doi.org/10.1016/j.cvsm.2013.04.004
Mealey, K.L., Bentjen, S.A., Gay, J.M., Cantor, G.H., 2001. Ivermectin sensitivity in collies is associated with a deletion mutation of the mdr1 gene. Pharmacogenetics and Genomics 11, 727–733.
Mealey, K.L., Bentjen, S.A., Waiting, D.K., 2002. Frequency of the mutant MDR1 allele associated with ivermectin sensitivity in a sample population of collies from the northwestern United States. Am J Vet Res 63, 479–481. https://doi.org/10.2460/ajvr.2002.63.479
Merola, V., Khan, S., Gwaltney-Brant, S., 2009. Ivermectin toxicosis in dogs: a retrospective study. J Am Anim Hosp Assoc 45, 106–111. https://doi.org/10.5326/0450106
Muhammad, G., Abdul, J., Khan, M.Z., Saqib, M., 2004. Use of neostigmine in massive ivermectin toxicity in cats. Vet Hum Toxicol 46, 28–29.
Petritz, O.A., Chen, S., 2018. Therapeutic Contraindications in Exotic Pets. Veterinary Clinics of North America: Exotic Animal Practice, Therapeutics 21, 327–340. https://doi.org/10.1016/j.cvex.2018.01.004
Qu, J., Li, M., Zhao, F., Liu, C., Zhang, Z., Xu, S., Li, S., 2015. Autophagy is upregulated in brain tissues of pigeons exposed to avermectin. Ecotoxicol Environ Saf 113, 159–168. https://doi.org/10.1016/j.ecoenv.2014.12.002
Robinson, I., Routh, A., 1999. Veterinary care of the hedgehog. In pract. 21, 128–137. https://doi.org/10.1136/inpract.21.3.128
Romero, C., Sheinberg Waisburd, G., Pineda, J., Heredia, R., Yarto, E., Cordero, A.M., 2017. Fluralaner as a single dose oral treatment for Caparinia tripilis in a pygmy African hedgehog. Veterinary Dermatology 28, 622-e152. https://doi.org/10.1111/vde.12465
Széll, Z., Sréter, T., Varga, I., 2001. IVERMECTIN TOXICOSIS IN A CHAMELEON (CHAMAELEO SENEGALENSIS) INFECTED WITH FOLEYELLA FURCATA. zamd 32, 115–117. https://doi.org/10.1638/1042-7260(2001)032[0115:ITIACC]2.0.CO;2
Teare, J.A., Bush, M., 1983. Toxicity and efficacy of ivermectin in chelonians. J Am Vet Med Assoc 183, 1195–1197.
Tranquilli, W.J., Paul, A.J., Seward, R.L., 1989. Ivermectin plasma concentrations in collies sensitive to ivermectin-induced toxicosis. Am J Vet Res 50, 769–770.
Wolstenholme, A.J., 2012. Glutamate-gated Chloride Channels. J Biol Chem 287, 40232–40238. https://doi.org/10.1074/jbc.R112.406280
Wolstenholme, A.J., Rogers, A.T., 2005. Glutamate-gated chloride channels and the mode of action of the avermectin/milbemycin anthelmintics. Parasitology 131 Suppl, S85-95. https://doi.org/10.1017/S0031182005008218
Wright, D.J., 1987. Avermectins: action on target pest species. Biochem Soc Trans 15, 65–67. https://doi.org/10.1042/bst0150065
Wright, H.M., Chen, A.V., Talcott, P.A., Poppenga, R.H., Mealey, K.L., 2011. Intravenous fat emulsion as treatment for ivermectin toxicosis in three dogs homozygous for the ABCB1-1Δ gene mutation. J Vet Emerg Crit Care (San Antonio) 21, 666–672. https://doi.org/10.1111/j.1476-4431.2011.00687.x